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double immunofluorescence labeling for piezo1  (Proteintech)


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    Proteintech double immunofluorescence labeling for piezo1
    RtPA upregulates <t>Piezo1</t> expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.
    Double Immunofluorescence Labeling For Piezo1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/double+immunofluorescence+labeling+for+piezo1/Piezo1+(extracellular+domain)+Antibody/pmc11218914-96-0-6
    Average 96 stars, based on 373 article reviews
    double immunofluorescence labeling for piezo1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator"

    Article Title: Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.14825

    RtPA upregulates Piezo1 expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.
    Figure Legend Snippet: RtPA upregulates Piezo1 expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.

    Techniques Used: Expressing, Labeling, Immunofluorescence, Staining

    Related Articles

    Immunofluorescence:

    Article Title: Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator
    Article Snippet: .. Double immunofluorescence labeling for Piezo1 (15939‐1‐AP, Proteintech, USA) and neutrophil cytosolic factor 4 (NCF4) (14648‐1‐AP, Proteintech, USA) was performed, followed by incubation with the secondary antibody and DAPI. .. Human Brain Microvascular Endothelial Cells (HBMECs) and human neutrophil‐like HL‐60 cells (ATCC, Rockville, MD, USA) were co‐cultured in a transwell chamber.

    Labeling:

    Article Title: Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator
    Article Snippet: .. Double immunofluorescence labeling for Piezo1 (15939‐1‐AP, Proteintech, USA) and neutrophil cytosolic factor 4 (NCF4) (14648‐1‐AP, Proteintech, USA) was performed, followed by incubation with the secondary antibody and DAPI. .. Human Brain Microvascular Endothelial Cells (HBMECs) and human neutrophil‐like HL‐60 cells (ATCC, Rockville, MD, USA) were co‐cultured in a transwell chamber.

    Incubation:

    Article Title: Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator
    Article Snippet: .. Double immunofluorescence labeling for Piezo1 (15939‐1‐AP, Proteintech, USA) and neutrophil cytosolic factor 4 (NCF4) (14648‐1‐AP, Proteintech, USA) was performed, followed by incubation with the secondary antibody and DAPI. .. Human Brain Microvascular Endothelial Cells (HBMECs) and human neutrophil‐like HL‐60 cells (ATCC, Rockville, MD, USA) were co‐cultured in a transwell chamber.



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    double immunofluorescence labeling for piezo1  (Proteintech)
    96
    Proteintech double immunofluorescence labeling for piezo1
    RtPA upregulates <t>Piezo1</t> expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.
    Double Immunofluorescence Labeling For Piezo1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/double+immunofluorescence+labeling+for+piezo1/Piezo1+(extracellular+domain)+Antibody/pmc11218914-96-0-6
    Average 96 stars, based on 1 article reviews
    double immunofluorescence labeling for piezo1 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    RtPA upregulates Piezo1 expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Neutrophil migration participates in the side effect of recombinant human tissue plasminogen activator

    doi: 10.1111/cns.14825

    Figure Lengend Snippet: RtPA upregulates Piezo1 expression in the cerebral microvasculature of the rats with MCAO. (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells. *** p < 0.001 vs Sham group, ## p < 0.001 vs MCAO group, ### p < 0.001 vs MCAO group. Results are shown as mean ± SD, n = 6 (one‐way ANOVA). (A) Double labeling immunofluorescence results of Piezo1 and NCF4 in the cerebral microvasculature of rats with MCAO after 72 h of reperfusion were obtained. The green channel represents Piezo1 immunofluorescence staining, the red channel represents NCF4 immunofluorescence staining, and the blue channel represents DAPI staining of the cell nucleus. (B) The intensity of immunofluorenscence of Piezo1. (C) Quantification of NCF4 positive cells.

    Article Snippet: Double immunofluorescence labeling for Piezo1 (15939‐1‐AP, Proteintech, USA) and neutrophil cytosolic factor 4 (NCF4) (14648‐1‐AP, Proteintech, USA) was performed, followed by incubation with the secondary antibody and DAPI.

    Techniques: Expressing, Labeling, Immunofluorescence, Staining